Pectic-β(1,4)-galactan, extensin and arabinogalactan–protein epitopes differentiate ripening stages in wine and table grape cell walls

Localization of Pectic Galactan in Tomato Cell Walls Using a Monoclonal Antibody Specific to (1[->]4)-[beta]-D-Galactan.

To develop antibody probes for the neutral side chains of pectins, antisera were generated to a pectic galactan isolated from tomato (Lycopersicon esculentum) pericarp cell walls and to a (1[->]4)-[beta]-galactotetraose-bovine serum albumin neoglycoprotein. The use of these two antisera in immunochemical assays and immunolocalization studies indicated that they had very similar specificities. A...

Localization of Pectic Galactan in Tomato Cell Walls Using a Monoclonal Antibody Specific to (1 --+4)-P-~-Galactan’

To develop antibody probes for the neutral side chains of pectins, antisera were generated to a pectic galactan isolated from tomato (Lycopersicon esculentum) pericarp cell walls and to a (1 +4)-pgalactotetraose-bovine serum albumin neoglycoprotein. l h e use of these two antisera in immunochemical assays and immunolocalization studies indicated that they had very similar specificities. A monoc...

Pectic epitopes are differentially distributed in the cell walls of potato (Solanum tuberosum) tubers

except at corners and pit fields. In contrast, pectic side-chains Six monoclonal antibodies (mAbs) were used to map the were not detectable in the middle lamella of all parenchymal distribution of pectic epitopes in the cell walls of potato cell walls, except in the cortex where mAb LM6 (arabinan (Solanum tuberosum L. cvs Kardal and Karnico) tuber tissue epitope) labelled the entire wall. The g...

Pectic Metabolism of Growing Cell Walls.

Solubilization of covalently bound extensin from capsicum cell walls.

Acidified sodium chlorite cleaves isodityrosine and solubilizes covalently bound hydroxyproline-rich material from cell walls. This has been taken as evidence that isodityrosine acts as a cross-link holding the hydroxyproline-rich glycoprotein extensin in the cell wall. However, acidified chlorite was found to cleave peptide bonds in salt-soluble extensin and in bovine serum albumin (BSA). This...